Journal: Heliyon

Article Title: Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans

doi: 10.1016/j.heliyon.2024.e40752

Figure Lengend Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™ on native human saliva samples alter protein yield. Representaive 2D gel electrophoresis results of untreated ( a ) and ReadyPrep 2-D Cleanup kit™, ReadyPrep Reduction-Alkylation kit™ treated ( b ) native human saliva samples (no RNAlater™ treatment) demonstrate both procedures alter protein yield primarily at the low molecular range. Highlighted areas (1–3) indicate identical regions of the separation. Proteins were visualized by silver stain.

Article Snippet: Urea, glycerol and mineral oil were obtained from Sigma, thiourea, sodium thiosulfate, bromophenol blue, glycine from Reanal, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), Bio-Lyte, dithiothreitol, iodoacetamide, Silver Stain Plus Kit and Bio-Safe Coomassie G-250 Stain from Bio-Rad, and SDS from Serva.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Silver Staining

Journal: Oncology Letters

Article Title: ZAP70 interaction with 13 mRNAs as a potential immunotherapeutic target for endometrial cancer

doi: 10.3892/ol.2023.13799

Figure Lengend Snippet: In vivo and in vitro validation of ZAP70 expression and its potential role in prognosis. (A) ZAP70 mRNA expression was detected in tissues by reverse transcription-quantitative PCR. (B) ZAP70 protein expression was detected in tissues by immunohistochemistry. Image intake was presented through the microscope (cat. no. 703548; Nikon Corporation). (C) Localization of ZAP70 in cells was detected using immunofluorescence (400×). Image intake was presented through the microscope (cat. no. 703548; Nikon Corporation). Differences in ZAP70 between (D) healthy and tumoral tissues, (E) FIGO clinical stages I/II and stages III/IV, and (F) living and deceased patients. IHC, immunohistochemistry; OS, overall survival; TPM, transcripts per kilobase million; ZAP70, ζ-chain-associated protein kinase 70. *P<0.05 and ***P<0.001.

Article Snippet: The 2-step plus Poly-HRP Anti Rabbit/Mouse IgG Detection System immunohistochemistry kit (cat. no. E-IR-R211; Elabscience Biotechnology, Inc.) was used to analyze tissue sections (4 μm).

Techniques: In Vivo, In Vitro, Biomarker Discovery, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Immunohistochemistry, Microscopy, Immunofluorescence